Serial Dilution Calculator

Number of dilutions

12 8 6

Replicates per dilution

Volume per replicate

Stock concentration

Highest concentration

Lowest concentration

Dilution factor

Step 1: Dilute stock to

Buffer volume:

Stock volume:

Total volume:

Aliquot of diluted stock into replicate wells

Step 2: Perform dilution series

Aliquot buffer into x wells:

On each dilution, move:

Intermediate volume: (check well capacity)


Dilution factor: