Use fluffyfrog's serial dilution calculator to set up for dose response assays in the lab. Say you want 12 dilutions between 1uM and 100uM _ ff's serial dilution calculator will calculate the 12 concentrations that can be produced using a serial dilution and report the volume of each diluent to transfer on each subsequent dilution. Replicate samples can also be included in volume calculations. Serial dilutions space out concentration points on log scale much better, making for better curve fit and more accurate EC50 or IC50 calculation. Fluffyfrog's serial dilution calculator makes planning experiments, especially in 96 well or 384 well plates, ridiculously easy.
Buffer volume:
Stock volume:
Total volume:
Aliquot of diluted stock into replicate wells
Aliquot buffer into x wells:
On each dilution, move:
Intermediate volume: (check well capacity)
Dilution factor:
